Processing
5' 6-FAM (Fluorescein)
Return to Fluorophores ModificationsA single isomer derivative of fluorescein. FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5. FAM can be attached to 5' or 3' end of oligos.
Scientific Details
| MW: | 537.5 |
| Mod Code: | /56-FAM/ |
| Extinction Coefficient: | 20960 |
| Absorbance Max: | 495 nm |
| Emission Max: | 520 |
| Extinction Coefficient (At Absorbance max): | 75000 |
Pricing Details
| Product | Pricing | Order |
|---|---|---|
| 100 nmole DNA Oligo | $82.00 USD | Add To Order |
| 250 nmole DNA Oligo | $103.00 USD | Add To Order |
| 1 umole DNA Oligo | $218.00 USD | Add To Order |
| 2 µmole DNA Oligo | $268.00 USD | Add To Order |
| 5 umole DNA Oligo | $473.00 USD | Add To Order |
| 10 umole DNA Oligo | $885.00 USD | Add To Order |
| 100 nmole RNA Oligo | $82.00 USD | Add To Order |
| 250 nmole RNA Oligo | $103.00 USD | Add To Order |
| 1 umole RNA Oligo | $218.00 USD | Add To Order |
| 5 umole RNA Oligo | $473.00 USD | Add To Order |
| 10 umole RNA Oligo | $885.00 USD | Add To Order |
| 100 nmole DNA Plate Oligo | $41.00 USD | Add To Order |
| 250 nmole DNA Plate Oligo | $51.00 USD | Add To Order |
| 1 umole DNA Plate Oligo | $105.00 USD | Add To Order |
Absorbance and Fluorescence Emission
Structure
