Processing
3' JOE (NHS Ester)
Return to Fluorophores ModificationsJOE (6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein) is available only as an NHS ester and must be attached to an amino-modified oligonucleotide. JOE is frequently used in multiplex reactions with FAM.
Scientific Details
| MW: | 698.4 |
| Mod Code: | /3Joe_N/ |
| Extinction Coefficient: | 20100 |
| Absorbance Max: | 529 nm |
| Emission Max: | 555 |
| Extinction Coefficient (At Absorbance max): | 75000 |
Pricing Details
| Product | Pricing | Order |
|---|---|---|
| 100 nmole DNA Oligo | $245.00 USD | Add To Order |
| 250 nmole DNA Oligo | $245.00 USD | Add To Order |
| 1 umole DNA Oligo | $371.00 USD | Add To Order |
| 2 µmole DNA Oligo | $486.00 USD | Add To Order |
| 100 nmole RNA Oligo | $245.00 USD | Add To Order |
| 250 nmole RNA Oligo | $245.00 USD | Add To Order |
| 1 umole RNA Oligo | $371.00 USD | Add To Order |
| 100 nmole DNA Plate Oligo | $245.00 USD | Add To Order |
| 250 nmole DNA Plate Oligo | $245.00 USD | Add To Order |
| 1 umole DNA Plate Oligo | $371.00 USD | Add To Order |
| * Requires HPLC Purification | ||
Absorbance and Fluorescence Emission
Structure
Purification Compatibility
| Requires HPLC | Yes |
| Requires IE-HPLC | No |
| Allows PAGE | No |
| Requires RNase-Free HPLC | No |
