xGen™ NGS cfDNA & FFPE Workflow Base Kit
All-in-one targeted NGS workflow
Accelerate your oncology research sequencing with a complete xGen workflow, engineered for high sensitivity, uniform coverage, and reliable performance with challenging cfDNA and FFPE samples. This all-in-one “base kit” simplifies ordering, eliminates compatibility guesswork, and delivers consistent results, making it ideal for fast moving studies.
xGen NGS—made to accelerate discovery
Ordering
- One simple, streamlined kit
- Engineered for high sensitivity, uniform coverage, and reliable performance with challenging cfDNA and FFPE samples
- For translational oncology research, liquid biopsy, and FFPE-heavy research environments
- Compatible with xGen Hyb Capture panels including custom designed
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Get everything you need for high-quality targeted NGS in one simple, streamlined kit.
Our complete xGen™ NGS cfDNA & FFPE Workflow Base Kit removes the complexity of selecting compatible components and accelerates the path from sample to sequencing.
Built on trusted xGen technologies, it brings together validated components designed to work seamlessly—giving teams greater confidence in every step of targeted NGS.
Designed for both new and experienced users, this all‑in‑one solution makes it easy to get started with IDT. One product, one order, and one optimized workflow deliver the confidence that every component works together seamlessly. From challenging cfDNA and FFPE samples to demanding variant detection at ≤1% VAF, you’ll achieve uniform coverage, reliable enrichment, and outstanding on‑target performance—with a fast, automation‑friendly protocol that supports diverse throughput needs.
Start your targeted NGS workflow with complete clarity, reduced setup time, and proven xGen reliability—all in a single, convenient purchase.
Included in this kit:
| 10010207 | xGen cfDNA & FFPE DNA Library Prep v2 MC, 96rxn |
| 10025272 | xGen Hyb and Wash Beads v3, 16 rxn* |
| 10028311 | xGen Hyb and Wash Reagents v3, 16 rxn |
| 1077675 | xGen Library Amplification Primer Mix, 16 rxn |
| 10005922 | xGen UDI Primers Plate 1, 8nt |
| 1075474 | xGen Universal Blockers TS, 16 rxn |
*Shipped separately
- This kit supports processing of up to 96 samples in a 6-plex reaction. Review the Getting Started Guide for full details on experimental set up
- Compatible with xGen Hyb Capture panels including custom designed
- One simple, streamlined kit
- Engineered for high sensitivity, uniform coverage, and reliable performance with challenging cfDNA and FFPE samples
- For translational oncology research, liquid biopsy, and FFPE heavy research environments
- Compatible with xGen Hyb Capture panels including custom designed
- Quality documentation is available for individual components. To request documentation, please reference the catalog number and lot number for each component.
Resources
Frequently asked questions
Will all components in the xGen™ NGS cfDNA & FFPE Workflow Base Kit be shipped together?
This workflow kit contains the following products:
|
Catalog Number |
Product Name |
|
|---|---|---|
|
10010207 |
xGen™ cfDNA & FFPE DNA Library Prep v2 MC, 96 rxn |
|
|
10025272 |
xGen™ Hyb and Wash Beads v3, 16 rxn |
|
|
10028311 |
xGen™ Hyb and Wash Reagents v3, 16 rxn |
|
|
1077675 |
xGen™ Library Amplification Primer Mix, 16 rxn |
|
|
10005922 |
xGen™ UDI Primers Plate 1, 8nt |
|
|
1075474 |
xGen™ Universal Blockers TS, 16 rxn |
These products will be shipped in two boxes, (1) Hyb and wash beads are temperature sensitive and ship room temperature, and (2) All 5 other components.
Upon receipt, please store as follows:
- Library prep kit reagents: -20°C (PEG/NaCl may be RT or -20°C)
- Hybridization buffers: Store at –20°C or 4°C per component (and bring to room temperature before use)
- Beads: Store at 4°C (do not freeze)
How do I order off the shelf panels vs. custom panels?
Off the shelf panels are ordered directly from IDT’s product/catalog pages for predesigned panels, while custom panels are ordered by designing them in the xGen™ Hyb Panel Design Tool or requesting a consultation with IDT to define specifications before ordering.
When should I purchase a custom hybridization capture research panel?
A custom panel is appropriate when predesigned options do not cover your targets or when you need flexibility to tailor, expand, or combine probe content across specific regions or application. For more information about custom panels, please reach out to IDT to discuss your specific needs.
What hybridization capture research panels does IDT offer?
IDT offers xGen™ predesigned hybrid capture research panels (e.g., Exome, AML Cancer, Inherited Diseases, Pan‑Cancer, CNV Backbone, Human ID, mtDNA, SARS‑CoV‑2) (xGen Predesigned Hyb Capture Panels) as well as xGen custom hybrid capture research panels (xGen Custom NGS Hybridization Capture Panels) that are built to user specifications and can be combined or expanded for DNA, RNA, or methyl‑seq studies. For more information about IDT’s amplicon sequencing solutions, please reach out to us here.
How many reactions and samples can I run using xGen™ NGS cfDNA & FFPE Workflow Base Kit?
This kit is configured to accommodate 96 samples in a 6 plex reaction but you are able to design your use as needed for your work. Please refer to the quick start guide and the individual protocols for more details.
Why is the xGen™ NGS cfDNA & FFPE Workflow Base Kit called a base kit?
xGen NGS cfDNA & FFPE Workflow Base Kit provides a complete workflow for preparing sequencing-ready libraries from cfDNA or FFPE DNA and performing hybridization-based target enrichment. This is called a base kit because it includes the reagents you need for (1) Library construction with dual in line UMIs, (2) Sample indexing using unique dual index (UDI) primers, and (3) Post-capture amplification for sequencing on Illumina® platforms. It does not include the xGen Hybridization capture panels that are defined specifically for your work. Those are available separately, ensuring you the flexibility to achieve success.